FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1802_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1802_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818900
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC1406.621958E-736.244137145
GCGAAAG9700.034.4203572
CGAAAGC9550.033.4151953
AGCGAAA9850.032.4501271
CTGCCGC6650.023.981083145
GCCGACC3102.4248948E-823.383314145
CCTATAC4659.094947E-1221.8358333
AGTCCGC2353.7159632E-521.592253145
TGATCCG1800.004312440320.1356329
ATACCGC3751.8132778E-719.3302066
GTATACG3101.4429617E-518.7468471
GTTACGT2000.00782841218.1610091
GTACGTA2400.001046133818.1610091
GATACCG4003.5684388E-718.124565
GATAGCG2901.868741E-417.49717145
TACGCAG2600.00181279616.7303645
GTATTAC7402.3646862E-1115.7068181
GTATAAG10300.015.5162031
GTGTAGG11250.015.4973941
GTGTACA9400.015.4561781
Produced by FastQC (version 0.11.5)