FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1802_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1802_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2311821
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA9400.030.1318231
GCGAAAG9900.028.5883312
TATACTG10850.026.7268585
CGAAAGC11850.023.8668673
GTGATCG3652.2009772E-1023.8324389
ACGACTC3052.0385414E-823.7693795
AGTCGAA2202.2137709E-523.0905742
GTTGACA15700.022.666531
GACGACT2953.8790677E-722.1185654
GTAATAT9700.021.7127531
GTCGAAG2455.1076462E-520.719593
CTACTGA25400.020.549336
AAGTCGA2505.8998437E-520.3351151
TAGTACC12900.020.2324854
TCGCACC2556.9627196E-519.901894
GTATATC4603.8562575E-918.945761
AGACGAC3451.7397597E-618.9178873
AAGACGA4806.5974746E-918.1425952
TAGTGCA12000.018.1225839
TACTGCG2400.001061232418.1225817
Produced by FastQC (version 0.11.5)