FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1802_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1802_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58412
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC640.1095665274258714No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGTA300.001926731672.498727
GTATAGA451.17958974E-464.498531
CCTCTAT350.003537923362.1950071
GACACAT350.00354996462.1417586
CTGTATA604.8863835E-448.3738981
TAAGGCT450.0095947748.3324784
ACATACA1102.408563E-432.953968
ATTCAGT950.004691729330.5257766
GTGTGGG1550.001779544723.4067231
GGGGGGG57450.09.969362145
AGGGGGG3007.1611383E-46.283222130-134
AAAAAGG3100.0070578165.612804125-129
Produced by FastQC (version 0.11.5)