FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1802_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1802_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3354101
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC49590.14784885726458447No Hit
TATGCAAAGGCTCTTAAGAATGTACTATGAACAATTACACCCCAACAAGC37270.1111177033726772No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT36540.10894126324758856No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA34400.102561014113767No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT34210.10199454339627817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC3500.062.135258145
CGACTAT902.0534571E-856.381996145
TAGTCGC553.2220408E-452.720825145
GCCGACC3450.039.922653145
GTTGACA27900.039.8363461
ACATGGT33950.032.459895
GACATGG34250.031.9653154
GGTCCTA41800.031.0419319
GCGAAAG7650.030.3556732
GTGATCG5850.028.4999399
ATGGTCC48200.026.6205867
TTGACAT41250.026.5646632
GCAGTGC26050.026.4915961
GCGTTAA2507.8538505E-826.0960339
CATGGTC41950.026.094916
TGGTCCT51650.024.8413338
TATAGAC12050.024.6915362
ATCTATG24700.024.1162171
CAGTGCA29450.023.9021932
GTATTAT34500.023.3720131
Produced by FastQC (version 0.11.5)