FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1801_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1801_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600793
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG6350.043.4364432
AATCGCT2005.456968E-1239.870396145
AGCGAAA7150.038.5981561
CGAAAGC7400.038.2217643
CCGACTA1652.3874345E-630.7578684
GTGATCG1652.3897628E-630.7540239
GTAGCGA1306.4878585E-427.8822676
TTACGTG2653.744206E-621.9123272
GTATTAT19150.020.858491
TATACTG9450.020.7138355
CTACTGA17350.020.473786
GTTACGT3208.337647E-720.4259131
GCTATAT4402.217348E-919.8069461
TAGCATG14150.019.492682
TAGGACT10500.019.3335174
TACACCG2256.8711536E-419.3329125
ACGGTAC2450.00120332317.785831
TATACAT13100.017.7095385
GTATAAC7400.017.6656551
GAGCGAA3352.8178061E-517.3433951
Produced by FastQC (version 0.11.5)