FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1801_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1801_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2433537
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT31410.1290713886823993No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC29430.12093508337863777No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAC1803.2086973E-936.244156145
AGCGAAA10950.032.501131
GCGAAAG11550.030.1603342
TAATGCG1000.006074222729.0018774
CGAAAGC12100.028.7681053
GCCGACC2654.5729394E-927.354078145
CCGACTA3709.094947E-1225.4746234
CTGCCGC10600.024.61867145
TTAGGTA10150.021.4299584
TATACTG12550.021.3741285
GTTACGT2755.1353854E-621.128751
GTAACCC2806.0819384E-620.7198853
CTATGCG8150.020.4614494
TATAGAC10400.020.2367862
CTACTGA23300.019.912186
GTATTAG7400.019.6297511
GCACCGT28100.019.0906266
ACCGTGT28500.019.075878
AGCACCG28450.018.6024885
TAATACG1950.00684075218.5909464
Produced by FastQC (version 0.11.5)