FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1801_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1801_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927375
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT30180.15658603022245282No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC26220.13603995071016278No Hit
GTGCTATGTGATCCATTCCCTCCTTCCTGAACTGGCAATCCAGATAAAGG20310.10537648355924509No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC20200.10480575912834814No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC1850.054.85597145
GTTACGT2700.045.7356721
TTACGTG2950.041.8249742
GCGAAAG8250.034.30992
CTGCCGC6550.034.307415145
AGCGAAA8400.033.725251
GTGCGAT1301.657706E-533.455259
GCCGACC1352.1456037E-532.217145
CGAAAGC9300.030.414873
ACGTGTT4150.029.6993774
GTTGACA11550.028.9298211
CCGACTA4200.027.6195874
GCGATTA1358.093107E-426.8475145
GGTGCGA1658.4333275E-526.3586798
ACATGGT13400.024.8866085
TATACTG10100.024.4045165
GCCGAGT4600.023.636868
TTAACGC1550.001810802423.383305145
TATAACG4053.274181E-1123.2702475
TACGTGT5300.023.263623
Produced by FastQC (version 0.11.5)