FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1801_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1801_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953840
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT33150.16966588871146052No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC32440.16603201899848505No Hit
GTGCTATGTGATCCATTCCCTCCTTCCTGAACTGGCAATCCAGATAAAGG26110.13363427916308399No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC22030.11275232362936577No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT3200.045.4177131
ACCGTAC803.6851445E-545.4177131
TTACGTG3400.042.6978872
CGCAATT700.001057619641.4227527
GCCGACC1859.640644E-1139.18368145
GACGACT1701.8299033E-938.384824
GATAGCG3400.036.244907145
ACGTGTT4250.034.119844
CCGACTA4050.032.2242934
ACGCAAT900.003631114532.218526
CGATGTT1652.3886787E-630.7564035
AGTCCGC1902.0808693E-730.522026145
GTGCGAT1453.4954333E-529.9957859
AGACGAC2451.9663275E-929.602833
TACGTGT4950.029.3038143
AGCGAAA6700.028.1996541
CGGAGCG1306.490508E-427.8807145
GTTATAG2104.8903894E-727.683181
GTTGACA11150.027.372831
CGTTAAC1606.724394E-527.2506281
Produced by FastQC (version 0.11.5)