FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1801_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1801_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3087906
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC76900.24903607817077333No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC42310.1370184196021511No Hit
ATGCTACACGATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTC40690.1317721459137681No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT39500.12791840166119045No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC39040.12642871900893354No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA38800.12565149327732125No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC36650.11868884609829444No Hit
ATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTCATGAAGCTTA33880.10971836577926919No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC2850.071.21462145
CGACTAT753.7687096E-757.989056145
GCCGACC3400.051.166813145
GCGAAAG7450.040.923082
CTGCCGC7850.038.78249145
GTTGACA23000.038.5411
ACATGGT22700.038.3262065
CGAAAGC8600.033.735873
AGCGAAA8650.033.599661
GTATTAT28100.032.321741
GCGTTAA2101.4446414E-831.0655659
ACGCCCG950.004733175530.5205559
GGTCCTA37450.030.1945679
GACATGG29250.029.7456844
GTAATAT10050.029.6420881
TGGTCCT37800.029.5314648
GACGACA2853.237801E-1027.977175145
CAGTGCA21900.027.8426462
TATACTG22450.027.7729845
CTTATAT13900.027.7046131
Produced by FastQC (version 0.11.5)