FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1800_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1800_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1422485
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGCG450.00964380748.32846145
CGAAGCG604.948252E-448.328469
GCCGACC2901.2732926E-1129.996973145
GTTACGT1753.7235568E-629.052221
CGATAAC1255.157966E-428.997074145
GCGAAAG6650.028.3729272
ATACCGA1306.4881303E-427.8818046
AGCGAAA6650.027.304721
CGAAAGC7050.025.7156683
CCGACTA3201.2423698E-924.921994
CTGCCGC5950.024.36729145
TTACGTG2101.5442152E-524.1897372
TACTGCG2751.9717481E-723.7248827
GAGCGAA2552.6664238E-622.7860551
GTATTGA6250.022.0796871
CGTATAC1850.00504059219.5995083
GTATAAT7100.019.4363461
GAGTGCG1900.005892515619.0770247
ACGCGTG1900.005892515619.0770247
TAGTACT4253.2745447E-818.7647914
Produced by FastQC (version 0.11.5)