FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1800_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1800_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476110
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC1751.00641046E-733.155353
TAGTCGG1851.6464219E-731.3472358
ACGCGTG1050.007713285327.616367
CGTATAC1855.737673E-627.4818921
CCGACTA2405.2750693E-827.1876134
AATCGCT2456.451046E-826.629156145
AGTCCGC1701.0325395E-425.584875145
TATAACG3751.0913936E-1125.132595
ACTACGC2651.3534736E-724.6670341
GTTGACA8650.024.3501821
CTACGCA3001.686385E-824.196282
GCTACTA1500.001495752524.1634949
GCCGACC1851.8328722E-423.510426145
GCGAAAG6550.023.2727552
ACGTCCG3753.092282E-1023.1969579
GCTATAT2852.7327587E-722.9360161
TAGTACT4455.456968E-1222.8090864
TAACGTC3854.292815E-1022.5952017
AACGTCC3854.292815E-1022.5944378
ATAACGT4256.184564E-1122.175066
Produced by FastQC (version 0.11.5)