FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1800_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1800_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187882
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG1650.048.4241871
GCGAACG450.0096034548.379292
CCGACGC1502.6035195E-838.661076145
GCCGACC1354.9780647E-737.58716145
CGAACGC800.00203316636.2630773
CTACGCA1854.1854946E-935.268144
ACTACGC1707.69669E-834.181781
GATAGCG2154.8748916E-1033.716057145
CCGACTA2653.6379788E-1232.8282054
AGCGAAA4250.030.7636031
CTACTGA14850.030.7531286
CAGTGCA6300.028.7971972
TATTGGG7900.028.4764212
AGACGAC1555.4877746E-528.074643
ACGACTC1555.5067016E-528.060465
AGTGCAG7000.027.9743733
GCTACTA1606.837883E-527.1824259
AGTCCGC1358.090439E-426.847971145
CTGCCGC4800.025.673372145
CGACTCC1701.0324664E-425.5845366
Produced by FastQC (version 0.11.5)