FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1800_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1800_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466007
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA52080.21119161462234293No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT47500.19261908015670676No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG43350.175790255258805No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA42540.17250559304981697No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA41730.16922093084082893No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA37120.15052674221930434No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG35670.14464679135136274No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT33950.13767195308042518No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC32320.1310620772771529No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC26570.10774503073186735No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA955.456968E-1268.675644145
GCCGACC4000.048.931396145
GTAGAAA25850.045.765642
ACCGTAC1307.223207E-944.7095381
AAGGTCG10050.043.2781839
ACTACGC2450.038.5505681
TATACTG34100.037.8429265
GGTACTG11500.037.8244365
TACTGAT10800.037.5879067
GTACTGA11750.036.4019286
AGCGAAA12100.034.2249641
GTATACG1707.686867E-834.1896441
CCTTATA32050.033.5155372
TTATACT35450.032.927894
CTACGCA2900.032.5355262
TATTCAA28350.031.9650965
ATACTGG35950.031.0550386
GGTCGTT10100.030.8624887
AGGTACT14400.030.7129554
TAGAAAC40200.030.1253993
Produced by FastQC (version 0.11.5)