FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1800_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1800_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2964291
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG258330.871473144843067No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA247920.836355135173976No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC211360.7130204153370907No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG191220.6450783678120671No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT173920.5867170260949415No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG165240.5574351505975628No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC163070.5501146817232181No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT145540.4909774377751712No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA130250.43939680685870586No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT129920.43828355583173173No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT124790.4209775625942257No Hit
TCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAATGTATTGATA124720.4207414184369888No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT122580.41352215420145993No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAA116800.3940233937896111No Hit
ATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTT110310.37212945692578764No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA101310.3417680652810402No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG99020.33404277785143227No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGT94570.3190307564270849No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC91880.309956073813266No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT90570.30553680458497495No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA87890.2964958568507613No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC85720.2891753879764166No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG83890.2830019050086513No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT80680.27217300865535804No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG78790.26579711640996107No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC73170.2468381140717966No Hit
TCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCA71850.2423851099639003No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA66880.2256188748000787No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT66030.2227514100336303No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA63780.21516106212244343No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA57560.19417796700796244No Hit
ATCTTACGAAAGGGGAGAAAGCTAATGTATTGATAGGGCAAGGAGACGTA57040.19242375326848815No Hit
GACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACACATCAG56380.19019725121454No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG56150.18942134898361868No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG55870.18847677235467097No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA55780.18817315843822352No Hit
CACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAA55230.18631774005993337No Hit
CTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGA53280.17973943853690477No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA52480.17704064816848278No Hit
GTTCATTGATGCTTAATGCTGGTCCATATCTTTTGTCTTCTTTGCCGAGG51900.17508402515137683No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG49030.16540211470466293No Hit
TCATAATCCTCGGCAAAGAAGACAAAAGATATGGACCAGCATTAAGCATC48920.1650310310290049No Hit
TATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGA48530.16371537072439918No Hit
GAGTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATG48120.16233224066058294No Hit
ACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTTAA46360.15639490185005453No Hit
TCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATC45520.15356117196321145No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC45260.1526840650934743No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA44920.15153707918689494No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAA43980.1483660005039991No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG43740.1475563633934725No Hit
GGATAGCAGTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGG43660.1472864843566303No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT43320.14613949845005095No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG43200.14573467989478767No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG43070.1452961264599191No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA42900.14472263350662942No Hit
GAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATACTGACAAA42830.14448648934939248No Hit
GTATTGAGAGGATTTCTAATCCTCGGCAAAGAAGACAAAAGATATGGACC42450.14320456392439204No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT42440.14317082904478676No Hit
CCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTGCTAGGGAAG42430.14313709416518147No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG42250.14252986633228654No Hit
TTACCAACACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCT41990.1416527594625494No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG41950.1415178199441283No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA41270.1392238481309696No Hit
CATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTTAAT41250.13915637837175907No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG40800.13763830878952169No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTC40570.13686240655860035No Hit
GGAGTAGAGTCTGCAGTATTGAGAGGATTTCTAATCCTCGGCAAAGAAGA38630.130317839915177No Hit
GATATGGACCAGCATTAAGCATCAATGAACTGAGCAATCTTACGAAAGGG38520.129946756239519No Hit
ATATGGAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA37810.12755157978754447No Hit
TCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACT37340.12596604044609655No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC37270.12572989628885964No Hit
ATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACAC37250.12566242652964907No Hit
CTTCTAAGTGTGTGCATATTGCAGCGAACTTATTTGTTTCAATTTTCGGG36970.1247178499007014No Hit
TCGTAAGATTGCTCAGTTCATTGATGCTTAATGCTGGTCCATATCTTTTG36670.12370580351254312No Hit
GGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAAGA36350.12262628736517432No Hit
GGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCG36070.12168171073622665No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA35990.12141183169938442No Hit
ATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGCCC35370.11932026916385739No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATAT35140.11854436693293609No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA34830.11749858566517254No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACT34700.11706003223030396No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACC34530.1164865392770143No Hit
ATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAACTAC34310.11574437192569825No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC34020.1147660604171453No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT33830.11412509770464506No Hit
CTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTG33590.11331546059411846No Hit
ACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCAATTGAG33340.1124720886039866No Hit
GTGGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAA33160.11186486077109165No Hit
CTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTC33010.11135883757701251No Hit
TGTATTGATAGGGCAAGGAGACGTAGTGTTGGTAATGAAACGGAAACGGG32710.11034679118885427No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA32440.10943594943951185No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT31740.10707450786714261No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG31560.10646728003424766No Hit
ACACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTA31410.10596125684016852No Hit
ATGCAATAAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCAT30800.10390342918424676No Hit
GTATAAGGGAATGTGGTACTTATTGCATTTTGCGCTGGCACTTTTAAGAA30790.10386969430464148No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG30400.10255403400003577No Hit
AAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTT30170.10177813176911443No Hit
CGCGAGATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAA30000.10120463881582477No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT29970.10110343417700893No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT29820.10059741098292982No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA25400.0103.456452
CAGGTAC27000.097.246853
GGTACTG28450.092.2438055
GTACTGA31400.084.496826
AGTAGAA49850.083.812951
AGCAGGT33450.079.070361
CTGATCC36450.072.5888069
ACTGATC40700.069.4615558
AGGTACT40150.067.716314
AGCGAAA6900.067.3968051
ACAAGGC31950.063.0736928
TACTGAT43850.061.3307957
TTGGACA40750.059.2467044
GCGAAAG7850.059.182462
TTTTTGG40050.058.9643141
CAAGGCA34300.058.9636549
GACAGTA40250.058.8919837
TTTGGAC41250.058.025753
ATAAAAG59500.056.684823
GAATAAA62950.054.366781
Produced by FastQC (version 0.11.5)