FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1800_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1800_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953547
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAA9900.10382288445142189No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC1300.061.334618145
GACGACA1307.36145E-944.606995145
CTGCCGC3750.038.659393145
CGAAAGC3300.032.9621773
CCGACGC1551.4670659E-632.73578145
GCGAAAG3400.032.033032
CTAGTAC1402.742201E-531.0786233
GTTGACA7950.030.1661911
AGCGAAA3700.029.4620651
CTACGCA1306.481904E-427.885224
CGGGAGT1306.481904E-427.885224
GGACGTG1306.4838916E-427.8837575
ACATGGT8250.027.2415875
TAGTACT2954.820322E-1027.0344854
GTATTAG3800.026.77431
AACGGGT1658.4049185E-526.3697413
TATTATC6100.026.1865652
GTATTAT8150.025.859141
GTAATAT3108.3491614E-1025.7872281
GTAACGG1701.01466314E-425.6493281
Produced by FastQC (version 0.11.5)