FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1797_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1797_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520734
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC16380.1077111447498379No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG15670.10304234665628571No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA7700.049.9945871
GCGATTA450.0096476748.323727145
GCGAAAG8050.047.778412
CGAAAGC8600.044.688983
TACTGAT7350.029.5869247
GGATAGC7850.029.5521475
TATACGA1000.00607450829.0009084
CTATACG1050.007702331527.6244533
ACTGATC10450.026.3583938
TATACTG10250.025.46175
GGTACTG8000.025.3732935
GACAGTA10200.024.1626557
AAGGTCG3902.0008883E-1124.1618619
CTGCCGC8200.023.867205145
GTACTGA10100.023.6841876
GCCGACC2653.7885893E-621.88244145
GATAGCA10700.021.6786446
TTATACT11400.021.6234844
GTATGGA12550.021.4139041
AGGTACT10200.021.3241984
Produced by FastQC (version 0.11.5)