FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1797_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1797_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1639453
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT22560.13760687253614468No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAC1351.0279109E-842.95693145
TACTTCG1209.578809E-636.24387
GCGAAAG6450.034.880492
ACCGTAC900.00359036532.2925451
AGCGAAA7200.031.2834031
CCGTACA1453.4675362E-530.0305882
TATACTG9250.029.7814185
CGAAAGC7600.029.5835513
CTGCCGC7700.029.184212145
GTATAGA15100.027.9084591
AATCGCT1855.82547E-627.42858145
TATAGAC17050.025.9648672
CCACGAC2051.2871422E-524.7654613
GATATAC3102.4105248E-823.395943
TAGTGCA17550.023.1299489
GTGTACA16350.022.6640341
GTAGTGC19100.021.6324278
GTACTGG4109.1495167E-1021.2658231
GGTAGTG18100.020.8251677
TAAGGTA18400.020.4912534
Produced by FastQC (version 0.11.5)