FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1797_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1797_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2465459
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG170970.6934611364455867No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT72360.2934950449388937No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG56490.2291256922139042No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT53510.21703869340354065No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC50520.20491113419448467No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA49920.20247751027293498No Hit
GTACATAATGAAGGGGGTTTACATAAATACAGCTTTGCTCAATGCATCTT42160.17100264088755887No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA41390.16787949018823675No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT41100.16670323862615438No Hit
GTTTAGAAATGTCTTGAGCATTGCCCCTATAATGTTCTCAAATAAAATGG38080.1544539982210209No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT37950.15392671303801847No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC36670.1487349820053791No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG36410.14768041163937426No Hit
GTATAGCATGGTGGGTTCTTGGGACCATTGGATCTTTACAGTCTCCCAAT35400.14358381137143225No Hit
ATATTGGCTTCTGGCAGCCTTTGGTACTAAAGATTGAAATGGTTCAAACT35020.14204251622111747No Hit
CCTTTACAGTTACCGGAGACAACACCAAATGGAATGAGAATCAGAATCCT34690.1407040230642651No Hit
TCTCTATACTTTCTCCTATTATAGGTCCCGTTCCGAATTGTTTCCATGCA33600.13628293960678317No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG33350.1352689296394708No Hit
CTTATTAGGTAGCTCTTTTTTGTCAGCTTTTGCTTCTTCTTCCCTATTGT31450.12756245388789675No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG31060.12598059833888944No Hit
GTCTAACAGCCAATGAGTCAGGAAGGTTAATAGATTTCCTCAAGGACGTA30780.12484490717549958No Hit
CCATAGAGGTATTCAGATCGAACGGTCTAACAGCCAATGAGTCAGGAAGG30390.12326305162649227No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG30060.12192455846963993No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC28710.11644890464615311No Hit
CAATATGATCAATAATAAGATTGATGACCAAATACAAGACGTATGGGCAT28610.11604330065922816No Hit
CTATTAACTCTTACATATTTGGGGCAGGTTCCAAATGCATATTTACTGAT28410.11523209268537826No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG28260.11462368670499083No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG28160.11421808271806588No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC27680.11227118358082613No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCACTTGGAGAA27210.11036484484227885No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC26580.10780953972465168No Hit
CCTATAATGTTCTCAAATAAAATGGCGAGGTTAGGAAAAGGATACATGTT26560.1077284189272667No Hit
ATACAAGACGTATGGGCATATAATGCAGAATTGCTAGTACTACTTGAAAA25870.10492975141748453No Hit
GTAAGGGAGAATTCCATACTCACAAAGTTCACCACGTCAGTATCATTTCT25860.10488919101879204No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC25370.10290173148285978No Hit
TCTATGATGTGGGAGATTAACGGGCCAGAATCAGTGCTAGTTAACACATA24990.10136043633254498No Hit
ATATAGAAGTATTGCATTTGACTCAAGGAACTTGCTGGGAGCAGATGTAC24850.10079259075085004No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG4500.093.440235
GCAGGTA4750.091.660332
CTGCCGC1900.080.1231145
AGTAGAA7200.075.657071
CTAGATA300.001936893172.492325145
TCTGACC2200.072.492325145
AGGTACT7900.056.898484
GTACTGA8150.054.2591556
CTTATAA3650.049.652275145
TTTAAAC5850.049.588523
AACACTG6350.049.0922785
TATAGCG8800.046.1773642
CTATTAG54450.045.8861121
GCGGTAT8950.045.359256
TATAGTA657.306641E-444.6550372
AGAGGTC56400.044.6007739
CCCTTAT6700.044.4457051
TATTAGT56700.044.1529662
AGAAACA16600.044.125573
TCGCCAT52100.043.41191145
Produced by FastQC (version 0.11.5)