FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1797_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1797_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2084721
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT30220.14495944541259959No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC1850.062.69381145
GCGAAAG6500.044.657252
AGCGAAA7750.037.503091
TATAGCG1209.507496E-636.2840162
CGAAAGC8800.032.963313
GTGCGAC3000.026.5904033
CTACGAC1400.001000652325.8891818
GTCGGTA9300.025.722155145
AAGGTCG4350.024.996459
TATACCG10550.024.7393885
GTATAGG10100.024.4605581
GCGGTAT2151.8680073E-523.6029996
ATAGCGG1902.1903307E-422.9008243
GCCGACC4557.2759576E-1222.304525145
GCGGACC1650.00260394221.966589
ATAGTCG13100.021.5809068
AGCGGTA2053.6682328E-421.2195555
AGGTACT12500.020.8815444
TACCGAG12200.020.7967287
ATACCGA12550.020.7953576
Produced by FastQC (version 0.11.5)