FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1796_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1796_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563697
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG7450.037.997052
AGCGAAA7300.037.8174171
CGAAAGC8750.032.3227963
TATACGG1601.8774335E-631.7200035
CCGTAGA1453.4853674E-530.0079023
ACCGTGC2202.2700988E-829.6536248
TATACCG1255.153962E-429.0011445
TTACGAG1804.6925597E-628.2018743
TCCGTAG1358.030184E-426.8830052
GTCCGTA1409.877858E-425.9461611
ATACCGT1400.001000227825.8905686
CTACGGA1701.0298116E-425.5949763
TACACCG1801.5204125E-424.1676225
GTACGTA1550.001787428323.435241
TACGGAC2202.2286124E-523.069834
GTATAGG2953.801215E-722.1641771
GTGTAGG9550.022.061031
TCTACGC1650.002597533621.9754853
ACGGACT2353.7097703E-521.59665
TAGGACT9550.021.2580644
Produced by FastQC (version 0.11.5)