FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1796_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1796_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298516
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG6500.039.083672
CGAAAGC7250.035.0162283
AGCGAAA7600.033.4526251
TCGCCAT1600.002178403322.651047145
GACTACG1650.002604163521.9654967
GAGCGAA3406.305345E-821.36471
CTGCCGC3757.788913E-921.261782145
TATACTG8800.020.5958255
TAGTGCA8150.020.4554239
GTATACT5800.020.0015664
GTATAGA12950.019.6324271
ACGGTCC1850.00505438319.5900949
GTATAGG3001.0876796E-519.3706631
ACCGTGC1900.00589663319.0745668
GTACTAG3151.6564114E-518.448251
ATTATAC9200.018.1334063
GCACCGT5802.0736479E-1017.4973286
GGTAGTG7500.017.3966737
GGGTTGA11600.016.8724236
GTATAAG3956.2224117E-616.550881
Produced by FastQC (version 0.11.5)