FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1796_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1796_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127400
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC1056.918526E-848.323677145
GCGAAAG5500.047.499842
AGCGAAA6200.042.176281
CGAAAGC6800.038.3934173
GCGGAGT900.003631976732.2157866
GAGCGAA2101.4139005E-831.1301121
TATACCG1000.00607807728.996785
CGGAGTT1100.00968151726.3583727
GCACCGT3955.9117156E-1022.0209186
TATATCG1800.00431017620.1366545
CTAGTAC4001.6267222E-819.9432493
GTAGGAC6900.018.9184953
GTATACG1950.006766987518.6248531
CTTAACG2901.8389084E-417.5330521
TAGCACC6404.5474735E-1116.9918084
TATACTG8650.016.7611455
AGCACCG5652.764864E-916.6795645
GTATATA7501.8189894E-1216.4643691
ACCGTGT4851.4828947E-716.4400168
TCTACAC8900.016.2968313
Produced by FastQC (version 0.11.5)