FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1796_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1796_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682579
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5700.049.6497842
TGACGCG450.00964573748.326397145
AGCACCG6900.047.280045
GCACCGT7250.041.9952246
ACCGTGT8550.038.152428
GTAACGC1406.6067696E-736.2544983
CTGCCGC7050.035.987743145
CCGACTA2252.0008883E-1135.4456834
CACCGTG9050.035.243567
CGAAAGC8400.033.664893
ACATAGC10250.031.177711
AGCGAAA9800.028.9037321
GTATTAG9600.028.7493321
CCGTGTT11600.028.7458749
TAGCACC12750.026.7264234
GCCGACC1907.1672166E-626.706694145
CCACCGA3652.1464075E-1023.878321
ATAGCAC14000.023.8243833
TATACTG14750.023.5919385
CGTAGAC1600.002174195422.659063
Produced by FastQC (version 0.11.5)