FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1796_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1796_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11399471
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT114940.10082924023404245No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG35750.054.1354269
CGAAAGC63400.033.1735043
CATAGTG62100.032.1065864
GCCGACC22100.029.191055145
GTCATAG73350.028.3968032
GTGATCC78300.026.10568
CTGCCGC57050.023.378372145
AGTGATC88150.023.1888317
CTTAGAC57350.023.0155073
GTGATCG9200.022.8484389
TAGTGAT94300.022.5236346
GACGAAC16600.022.26969145
AGCGAAA102050.020.9275741
GTAGTAT58800.020.8782421
GTATATA52850.019.7925641
TAGTATG43850.019.695412
GGTCATA123500.019.6455441
GCGAAAG112800.019.044532
TATGCTG92600.018.7893335
ATAGTGA116700.018.6363775
Produced by FastQC (version 0.11.5)