FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1796_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1796_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203230
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACG450.00964522548.3275459
AGCGAAA11100.044.49291
GCGAAAG11700.042.175742
CGAAAGC14850.034.1818543
TGATCCG4250.032.4078879
TATACTG11950.029.1211575
CGTTATA2101.5350388E-524.2093751
ACCGGTC1500.001495885724.163773145
GCCGACC3952.3646862E-1123.857903145
CTGCCGC7900.022.94029145
GTATAAG9300.021.8665311
CACGACG3551.0161784E-720.4224055
CCGACGC3601.1791599E-720.136478145
GTATTAG12050.019.8898581
GAGCGAA3501.9677864E-618.6758041
TATAACG2359.1926253E-418.5143053
TAGTGCA10250.018.388049
GCGGACC2450.001218534417.7529759
TACATTG9000.017.72017
GTACCGT3302.5098425E-517.5792393
Produced by FastQC (version 0.11.5)