FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1795_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1795_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1822411
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG8850.041.827182
AGCGAAA9150.038.1084941
TAAGGCG1501.1356915E-633.829063145
CGAAAGC11650.031.7490023
GTCGTAG1050.00763562627.6740261
GTACCGC1400.001000351925.89036
CTGCCGC7100.023.482952145
ACGTCCG2903.2931348E-722.4971629
GTCCATA6750.020.448031
GAGCGAA3301.1175161E-619.8120861
GGGCCGA4904.1472958E-1019.2327946
CACCGTT3852.3908797E-718.8287937
TACCGCA1950.006797925618.6108862
TATAACG4354.2797183E-818.3320275
TAACGTC4857.601557E-917.935887
GACTACG2450.001218476917.7528617
GTCTAAG9100.017.5623631
ACCGTTC3753.8710223E-617.3978048
GCCGAGT4205.957827E-717.2597268
GTCCTAT13700.016.9680161
Produced by FastQC (version 0.11.5)