FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1795_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1795_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545083
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG604.8903556E-448.4385531
GTGATCG1051.8539352E-434.519629
GCGAAAG1759.975156E-833.1814422
GAGCGAA950.00467542430.592771
CTTAGAC2502.4301698E-929.0124443
ACGGGAC1358.057746E-426.8633753
TACACCG1100.00967352426.3604355
CGAAAGC2502.2675795E-623.2099553
GTCCTAT4453.6379788E-1222.8586431
ATTAGAC2953.8517283E-722.1281383
GTAGTGT2003.1030792E-421.747366
GTATATA4709.094947E-1221.6427571
GTCTAGG1750.003610831720.7593771
AGCGAAA2805.9684735E-620.7593771
GTCTTAG2856.956114E-620.395181
GCACCAT1850.005047532719.5922136
TATACTG4452.6011548E-919.5481855
TTAGACT2256.8678026E-419.3309864
GCACCGT2256.8678026E-419.3309866
ATAGCAC4603.8999133E-918.9211623
Produced by FastQC (version 0.11.5)