FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1795_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1795_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495074
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC1004.7097274E-850.744102145
AGCGAAA5000.039.2333641
CCGACGC1859.640644E-1139.18463145
GCGAAAG6500.031.2626882
AGATCGT1902.0792686E-730.523796
TATACTG9000.028.1939965
CGAAAGC7150.027.3835963
CTGCCGC8200.026.521307145
AAGATCG1958.759356E-626.0252275
ATCGTGC2603.2100925E-622.3043548
CCGTAAG2653.712852E-621.9333971
TATGCGG2857.1028007E-620.3505545
TATTACG1800.00428265320.1594722
TATAATA14850.020.0372922
GTATAAT8300.019.2577861
TAACCGT1950.006842730618.5894475
CTAACCG2801.4264882E-418.1253174
GCACCGT8850.018.0211076
GTGATCG3302.5164762E-517.5731289
CGTAAGA2901.8516298E-417.5178852
Produced by FastQC (version 0.11.5)