FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1795_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1795_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932667
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC33820.1749913461553387No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC26100.1350465444900751No Hit
ATGCTACACGATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTC22000.11383233635178745No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT20750.10736458996816317No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA20130.10415658776188551No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCGC5700.045.781494145
GTGATCG1406.622904E-736.243689
GCCGACC2650.035.55984145
CGAAAGC8000.032.6387333
GCGAAAG8150.032.0554432
AGCGAAA8550.030.5866761
TATACTG11150.029.9089685
CCGACGC1753.7821446E-628.994944145
CCGTATA1555.466984E-528.0916022
CAATTCG3357.4578566E-1125.9656229
GTATACG2701.619319E-724.214451
TAGTACT6650.023.9863664
GTATATA11150.022.1513371
GGTAGTG9350.021.7074457
TCGCCAT4058.094503E-1021.477737145
GAATAAC13000.021.234211
TAGTATA5600.020.7342762
GAGTCGT1750.003662548520.7117466
GTATTAG8550.020.3911171
GCACCGT8250.020.2096426
Produced by FastQC (version 0.11.5)