FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1795_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1795_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244018
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG8000.036.292252
GCCGACC3100.030.400679145
CGAAAGC10150.029.2950123
TAGTACG1255.156369E-428.999514
GACGAAC2303.4946424E-828.36719145
AGCGAAA10650.026.6057621
CTGCCGC8650.025.980484145
AGCACCG7700.020.7125515
ACCGTAC1800.00431135920.1367538
GTATATA11700.019.2502571
TATACTA5801.0913936E-1118.748435
TTACGAG1950.006830622418.5956463
GCACCGT6750.018.2573226
CCGACTA2801.4282422E-418.123089
GTTTACG2851.6078004E-417.844891
GTCCTAT16850.017.6784691
TATACTG13650.017.5260055
GTATTAA31750.017.3912411
GTATTAG11350.017.2833771
GCGCACA3002.4205144E-416.9148739
Produced by FastQC (version 0.11.5)