FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1795_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1795_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531585
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAC1402.7102942E-1046.627523
AGCGAAA6400.037.46771
CGAAAGC7250.033.014433
GCGAAAG7150.032.4935842
GTGATCG1703.0166339E-629.8484429
GTTGACA9350.027.2006631
GACGACA1606.837476E-527.183405145
GTATAGG4200.025.9516531
TAACGAC1701.0285831E-425.5994223
GATAGCG1701.0327004E-425.58438145
GTTTACG2151.8341007E-523.658251
GTCCTAT12350.022.3583471
AGCACCG5403.6379788E-1220.137175
GCACCGT4402.2700988E-919.7703956
GGTCCTA12900.019.667589
AGGACCG2959.57472E-619.659345
TAATACG1850.00504675819.5954824
CCGACTA3351.3147746E-619.4784934
GCAGTGC10150.019.3295081
TATAGGG5305.638867E-1119.1781122
Produced by FastQC (version 0.11.5)