FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1794_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1794_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053027
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1056.913979E-848.327199
CGGAGTA2052.910383E-1035.368084
GTGCGAT850.002743760134.113319
GTTACGT1102.4116659E-433.0114941
CCGACTA3950.031.2044944
GCCGACC1000.006078373628.996317145
AGCGAAA3700.027.4798371
GAGCTAT4000.027.1840468
GTTGACA5750.026.5240151
ACGTGTT1658.415386E-526.3652954
GCGAAAG4001.8189894E-1225.4007242
CTGCCGC3151.035005E-925.314243145
GATAGCG2751.9712388E-723.724257145
TATACTG5900.023.344495
GAGAACG10000.023.1970527
CGAAAGC4455.456968E-1222.8147563
TTACGTG1650.002585693521.9919682
AATCGCT1650.002602869921.966906145
GGAGCTA5350.021.679497
CTACTGA13200.021.4177326
Produced by FastQC (version 0.11.5)