FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1794_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1794_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164955
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG14790.1269576936448189No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC12720.10918876694807954No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA12570.10790116356425784No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT12510.10738612221072917No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC11820.10146314664514937No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGCGA604.951857E-448.3205838
TTACGTG1307.2886905E-944.6592062
GTTACGT1351.009721E-843.0421521
GCGAAAG2850.035.6490142
GTTGACA7750.034.67671
GTGCGAT850.00274571934.108659
TATACTG6300.032.220645
TCGCCAT3700.031.343084145
TACGTGT1952.599263E-729.7549023
ACATGGT9300.029.62225
CGAAAGC3450.029.4314773
TAGCATG13800.027.345672
CTTAGAC3453.6379788E-1227.329233
CCGTCCG1358.0959813E-426.844777
CTACTGA12900.026.4088956
GCGTTAA1400.001001097725.886039
GTAGCAT15750.025.3641281
AGCGAAA3751.0913936E-1125.179661
ATCTATG6400.024.9678121
AGCATGT14650.024.7534373
Produced by FastQC (version 0.11.5)