FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1794_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1794_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2292540
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC212910.9287078960454344No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG210240.9170614253186422No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG149420.6517661632948607No Hit
GTCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA132050.5759986739598872No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG102220.445880987899884No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT91140.39755031537072416No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA82410.3594702818707634No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT81130.35388695508039114No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA68010.2966578554790756No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA62810.2739755903931883No Hit
GAATATAGGCTGTTGAACACCGTCAATGAAATGGAAGTCTGAATACATGA61770.2694391373760109No Hit
CACTTAGAAGTCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAA59530.25966831549285946No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG54990.23986495328325785No Hit
GAGTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATG53120.2317080618004484No Hit
GTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGGTGTTCAACAGCC52730.2300068919190069No Hit
ATGGAAGACTTTGTGCGACAGTGCTTTAATCCAATGATTGTCGAGCTTGC51670.22538319942072985No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA49880.21757526586231862No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG47070.20531811876782954No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT46210.2015668210805482No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC45790.19973479197745733No Hit
TTGTGCGACAGTGCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACA44070.19223219660289462No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA43930.19162152023519766No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG41810.1823741352386436No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT40490.17661632948607223No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA40430.17635461104277353No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA38380.16741256423006795No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA38090.16614759175412425No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT37780.16479537979708095No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG37680.16435918239158315No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC37510.16361764680223684No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA36990.1613494202936481No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG35970.15690020675757022No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT35740.1558969527249252No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC35600.15528627635722825No Hit
GCAATATGCACACACTTAGAAGTCTATATTCATCTCCACAACTCGAAGGA34920.15232013399984295No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG34640.15109878126444903No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG34060.14856883631256163No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC34050.14852521657201181No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG34050.14852521657201181No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT33840.1476092020204664No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATAT32370.14119710015964823No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC32210.14049918431085173No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA32190.14041194482975217No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGGTGT31530.13753304195346644No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT31280.13644254843972187No Hit
ATATGGAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA30530.13317106789848815No Hit
ATGTAGGACCATTCTCTTCCTCCCAACAGCAGGTCACAAGAAGGGTTGCC29600.12911443202735828No Hit
CAATATGATCAATAATAAGATTGATGACCAAATACAAGACGTATGGGCAT29330.12793669903251417No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG29020.12658448707547088No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG28960.12632276863217218No Hit
TCTCTATACTTTCTCCTATTATAGGTCCCGTTCCGAATTGTTTCCATGCA28880.12597381070777391No Hit
ACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCAATTGAG28490.12427264082633238No Hit
ACAGTGCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGG28400.12388006316138432No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT28320.12353110523698604No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCCAATGA28040.12230975250159212No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC27830.12139373795004667No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG27780.12117563924729775No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC27750.1210447800256484No Hit
ATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAACTAC27350.11929999040365707No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCACTTGGAGAA26840.11707538363561815No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC26610.11607212960297313No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG25750.11232083191569175No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG25620.11175377528854459No Hit
TTGTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTG25570.11153567658579568No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA25450.11101223969919828No Hit
GTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCG25370.11066328177479999No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA25360.11061966203425022No Hit
CTATTAACTCTTACATATTTGGGGCAGGTTCCAAATGCATATTTACTGAT24790.10813333682291258No Hit
GTGCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAAT24640.10747904071466584No Hit
GTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATGAA24550.10708646304971778No Hit
CTTAGAAGTCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATC24030.10481823654112905No Hit
ATAATAAGATTGATGACCAAATACAAGACGTATGGGCATATAATGCAGAA23660.10320430614078709No Hit
CTGCAATATGCACACACTTAGAAGTCTATATTCATCTCCACAACTCGAAG23480.10241915081089098No Hit
ATACAAGACGTATGGGCATATAATGCAGAATTGCTAGTACTACTTGAAAA23470.10237553107034118No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC22930.1000200650806529No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA29600.0111.2958152
ACGACAG203.8674584E-4108.7446445
GGTACTG31100.0106.063915
CAGGTAC34100.096.974993
GTACTGA36750.091.3276756
AGTAGAA13550.089.509941
TTGTACG259.3235995E-487.1515961
GTACGAC259.3787565E-487.022293
CTGCCGC3650.085.39931145
ATATCAC3250.078.096933
AGGTACT45200.077.152814
GTCCGGG300.001922736572.6263351
GTATCGA300.001922736572.6263351
ATCACTA3750.069.596585
TCTGACC1150.069.33836145
AGCAGGT49850.066.2888261
TTTCGTT451.1949927E-464.43565145
AACACTG8900.064.350775
TTTTTGG38400.062.4132581
TGTACGA350.003554855562.196782
Produced by FastQC (version 0.11.5)