FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1794_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1794_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2102489
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG118040.5614298100965094No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG30600.14554178404738383No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC30410.14463809323140334No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT30260.143924653113524No Hit
GTTTAGAAATGTCTTGAGCATTGCCCCTATAATGTTCTCAAATAAAATGG25560.12157019608663826No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT25550.12152263341211296No Hit
GTCTAACAGCCAATGAGTCAGGAAGGTTAATAGATTTCCTCAAGGACGTA23690.11267597595040926No Hit
CTTATTAGGTAGCTCTTTTTTGTCAGCTTTTGCTTCTTCTTCCCTATTGT23290.1107734689693977No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG21500.10225975022937099No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG7350.060.169675
GCAGGTA8250.054.5506632
CGAAAGC5050.047.3939633
CTATTAG37950.046.8916931
TATAGCG7450.045.7935032
TATTAGT40900.044.7238852
GTACTGA10300.044.3390966
TAGTAGA41450.042.6776435
GCGAAAG5450.042.6202282
GGATAGC11400.042.6093675
AGGTACT10600.041.723414
CAGGTAC10700.041.347243
TCGCCAT40150.039.540493145
AGAGGTC44900.039.3927889
ATTAGTA45900.039.344783
TATACTG10750.037.7671935
TTAGTAG49550.036.4343874
ACTGATC19000.035.8630688
TAGAGGT52200.033.7449658
GCGGTAT10150.033.5672156