FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1794_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1794_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1592421
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG27380.1719394557092628No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA18720.11755685211385682No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG16820.10562533400401025No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC1703.8198777E-1142.63801145
TCGCCAT8900.040.721695145
GCGAAAG5400.034.9274142
GGTACTG7200.034.2374465
TATTAGT15700.030.4951782
TATAGCG2901.2732926E-1130.0171952
GGATAGC5850.029.744755
CTGCCGC4200.029.339012145
AGGTACT8950.029.164984
TATATCG1000.006074365729.0011315
CTATTAG16650.028.777821
ACTGATC14400.028.1884618
CGAAAGC6600.027.4666443
GCGGTAT3453.6379788E-1227.3156176
AGAGGTC16300.026.6814549
GCAGGTA9000.026.598572
GTATAGG7150.026.3994871
GTACTGA10800.025.5062476
GCGTATA2001.0548081E-525.4095061
GTCGGGC1751.2547789E-424.8612353
Produced by FastQC (version 0.11.5)