FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1793_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1793_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349519
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5200.039.0981522
AGCGAAA5500.037.009481
CGAAAGC5650.035.946783
GAGCGAA2559.2450136E-825.6578351
ACCGTGC1500.001496739724.1605728
CGCAAGA2451.8876544E-623.7096632
CTGCCGC4205.2750693E-1122.434816145
GTATAGG2353.6334764E-521.6544841
TCGCAAG2104.2368265E-420.7706261
AGCACCG6800.020.2559955
ATACCGC3259.855248E-720.0726056
TATAGAC7300.019.8933832
ATAGTCG1900.005897518219.074135145
AATCGCT1900.005897518219.074135145
TAGTACT4253.273999E-818.7648414
TTACGTG2000.007848036518.1527122
GCACCGT7700.017.8857636
AGCCGTC2450.00121928517.7506247
TATACTG7850.017.5465955
ACCGTGT7550.017.2804098
Produced by FastQC (version 0.11.5)