FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1793_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1793_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985839
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC22140.11148940070166817No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC21400.10776301603503607No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC1803.2050593E-936.247135145
GCGAAAG7750.028.0891762
CGAAAGC8350.026.9221763
GATAGCG2401.600507E-624.164759145
GCACCGT8800.023.8895576
AGCGAAA9050.023.2723981
TATACTG9500.022.8929275
GTGGCGC1600.00215406122.6956061
AGCACCG9500.022.129835
TAGTACT4607.2759576E-1222.0656974
CTGCCGC6700.021.640081145
GTTACGT2053.6254484E-421.2563721
GTATAGG4451.10958354E-1021.2165661
TACACCG2404.3683252E-521.1441635
CACCGTG12500.020.2978847
GATATCG1800.004311141620.1367919
ATAGCAC15450.019.7131373
ACTATGC6500.018.9595628
GAGCGAA3451.7150505E-618.9458961
ACCGTGT11900.018.2754088
Produced by FastQC (version 0.11.5)