FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1793_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1793_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467062
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1602.0008883E-1145.367571
ATACCGT803.7331403E-545.3092886
GCGAAAG2250.041.9397961
AGGGCGA700.001056430441.4256366
AATACCG1001.3908942E-436.2474335
CGAAAGC2650.035.5787542
GCACCGT2003.2500793E-728.9979466
CCTATAC1050.007702952727.6200473
TATACTA1358.079572E-426.8499495
GTCTAGG1409.916044E-425.9243281
GTGCTAT2559.35288E-825.6193331
ACCGTGT2559.4700226E-825.5864228
AGCACCG2301.1016891E-625.2156035
GTATAAC1500.001482556724.196041
ATAGGAT1801.5182207E-424.1675423
TAGGATC1550.001807798223.3854394
CACCGTG2603.2000753E-622.306117
GTATACC1650.002598258221.9704913
TTGTCTA1650.002599874321.968149
CCGTGTT3106.2335494E-721.0468969
Produced by FastQC (version 0.11.5)