FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1793_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1793_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1920671
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG951.02971695E-438.15492145
GCGAAAG8100.037.6249282
AGCGAAA7950.037.4679831
TATACTG10400.035.551045
CGAAAGC8850.034.415753
TATAGCG1707.770177E-834.146992
GTTACGT2252.7634087E-829.0605371
GTAACGG3251.4515535E-924.5896821
TTACGTG2751.9542858E-723.747682
TATACTA7000.022.7845335
CGCTATA1600.00216502922.6757352
CTGCCGC4800.022.654484145
TATACCG2603.208157E-622.3065345
GATATAC4251.4679244E-920.4759223
GTTACCG3208.4874955E-720.3885069
GCGGTAT2857.109944E-620.3487636
GAGCGAA3601.15185685E-720.1809251
CTACTAG4752.746674E-1019.8400429
GTATAAG7050.018.5492761
CTATACT9300.017.9301094
Produced by FastQC (version 0.11.5)