FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1793_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1793_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1845756
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGC100.007085495145.040023
GCGAAAG7650.035.0950132
GTTCGAT2404.3655746E-1133.2239349
AGCGAAA8400.031.988421
GGTTCGA2351.2660166E-930.8462038
CGAAAGC9300.030.4116173
GCACCGT12300.029.4700986
AGCACCG13300.027.2550375
ACCGTGT13950.026.5012028
CCGACTA3052.0350853E-823.7725454
CTGCCGC5500.023.723536145
TATACTG13150.023.1553825
CACCGTG15500.022.9163677
TAACGTA3203.3902325E-822.655755
TAGCACC17700.022.5301934
CGTTATA2003.086724E-421.7683932
CCGTGTT16350.021.7244059
GTTATAC8050.021.6208743
ATAGTCG2755.2217256E-621.0875878
ATAGCAC18950.021.0480233
Produced by FastQC (version 0.11.5)