FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1793_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1793_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233669
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT12960.10505248976832521No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAT400.006055784454.36464145
GCGAAAG5250.038.6985052
TCGCCAT3650.035.74661145
GTTGACA9750.034.2652661
GCGTTAC850.002718713834.1776351
CGAAAGC6250.033.6499523
CCGACGC1301.6572545E-533.45516145
CGTTACA1102.4247315E-432.981682
GGATAGC3750.032.865735
AGCGAAA6600.030.8116551
GTTACGT1255.101799E-429.050991
GGTCCTA12700.027.967119
GGTACTG4700.027.7651675
GTACTGA6550.027.6687266
GTGATCG1358.0927956E-426.8467339
ACATGGT12500.024.939295
GACATGG12050.024.6703474
CTTATAT7350.023.7150921
TATACTG11350.022.994945
GGTTATA5100.022.7850881
Produced by FastQC (version 0.11.5)