FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1790_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1790_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321709
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC14960.11318679073835465No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA700.001057941241.4192145
GCGAAAG3800.040.114392
TAGTCCG1501.1362645E-633.825689
CGAAAGC5050.030.1599523
CGTAGAC1255.143295E-429.0110023
CGGCATA1504.406557E-528.993449
GCCGACC1050.0077178527.612799145
CTTAGAC5000.027.5604523
GCGTAAT1658.3127605E-526.4136281
CGCTAGT1400.0010009525.887001145
CCGCACT1500.001494519924.1666874
AGCGAAA6550.023.2883511
TAATACG2202.2294927E-523.06824
CCTACGC1600.002178380522.6511239
CCGTACC1650.002601286621.9697134
CGCACTG1700.00309533821.3211255
GTCCTAT12100.020.4105321
TATACTG9700.019.43085
CGTAATA2256.813453E-419.3567872
TCTACAC6500.018.968733
Produced by FastQC (version 0.11.5)