FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1790_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1790_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372779
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTG1504.4037624E-528.9962567
CACGCGT1555.5053693E-528.0619146
GCGAAAG5800.026.2695732
CCGACGC2351.3325407E-624.677664145
AAGCGTT2101.5571739E-524.163546145
AGCGAAA6550.023.2870751
GAGCGAA4154.3655746E-1122.7526631
CGCGTGT1650.002603300121.966868
GTCTAGG8700.021.7065621
CCTATAC3457.493691E-821.018673
CGAAAGC7300.020.8717162
GTATAGG3851.0299118E-820.7524261
GTGTACA10600.020.556651
TATACAG11700.020.4475675
ACCTAGT2856.98903E-620.3883511
ACACGCG2155.0593744E-420.2314195
CTATACA8300.020.0906944
GTCCTAC7250.019.0349861
TAGGACT12300.018.86214
AAGGCGC2751.2435102E-418.4528356
Produced by FastQC (version 0.11.5)