FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1790_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1790_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866342
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG4400.044.5442662
GCCGACC855.3452037E-542.641235145
AGCGAAA4650.040.6258931
GTGCGGA800.002037830736.245059
CGAAAGC5550.035.2837833
TCGATTA2901.1919838E-824.996588145
GACCGTG2751.970584E-723.7240337
ACCGTGC1900.005891910819.0763448
TAATACA13850.018.8465444
ATAATAC13900.018.7841723
TATAATA16200.017.9236182
TAGTACT3703.3946508E-617.6367994
TACAGCC9500.017.5502367
ATACAGC8850.017.2020346
GTTATAC5501.9190338E-917.142933
TATGGAC4701.0289841E-716.9745863
GTCCTAT5602.3810571E-916.8669991
ATAGGAC4307.5695243E-716.8669263
TATACTG5652.755769E-916.6820285
CTAGGAC5252.006891E-816.5777783
Produced by FastQC (version 0.11.5)