FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1790_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1790_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381123
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCT300.00193731572.4764569
GCGAAAG1405.456968E-1251.843712
AGCGAAA1801.8189894E-1244.407691
CGAAAGC1807.094059E-1140.301713
CCGACTA750.001481731438.6693274
GGGTAGC1406.4774395E-736.3335651
CTAGTAC800.002029110436.2715343
CACACCG1209.552408E-636.2477345
ACGTCCG1051.8551075E-434.5125969
GTACTAC850.00270910134.1962971
TCCGGCC900.003631207932.2117548
GCAGTGC3200.031.7918681
CTACAAC2951.4551915E-1129.5090473
CTATACA2003.2434582E-729.0019934
TATAACG1255.1498093E-428.998195
GCTACTA2003.2546632E-728.990589
ACACCGT1306.487846E-427.8755576
GTGTAAG3503.6379788E-1226.9906461
GAGTAGG1907.0135957E-626.7720991
CAGCTAC1907.1582817E-626.7018497
Produced by FastQC (version 0.11.5)