FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1790_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1790_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1826357
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG9300.042.1446652
TATACGG1209.572679E-636.2475135
GCCGACC2451.8189894E-1235.504856145
AGCGAAA11050.035.5032581
CGAAAGC12050.032.502533
CAATCCG1000.006081041428.9948399
CTGCCGC8300.026.20087145
TGGTACG1650.002601469421.9699954
GTATTAG9600.019.6760921
TATAATA19600.019.6268922
GTATAGA13050.018.9280191
ACTGTCG3451.7465918E-618.9096788
GACGAAC1950.00684862218.586943145
TAATACA27150.018.1586274
TACGGGG2952.1298381E-417.2003277
ATATACG2550.001591529517.0590554
ATAATAC20050.017.001763
ATACAGC15500.016.8361726
GTATTAT17300.016.7977311
GTGTAGG9150.016.6738131
Produced by FastQC (version 0.11.5)