FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1790_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1790_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911599
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAC800.090.653053
GCGAAAG5200.071.190292
ATCTACG350.00353192362.2954251
AGCGAAA6000.060.5651
CGAAAGC6700.055.203653
TCGCAGC450.00964588548.324425145
GATGCGC907.4900505E-540.2703558
CCGACGC1105.2704545E-639.53817145
TCGCAAC800.002038363436.24332145
GCCGACC850.002744336434.111362145
TTACTCG900.003628414132.221594
GACGGTA1153.1653006E-431.5159327
GCGGATA950.004670714530.6012611
ACGGTAG950.00473134130.520698
TACTCGG1204.0595385E-430.2060815
TGATCCG1952.6110865E-729.738119
AGCCGGC1000.006079648628.994667
CCGGTAA1000.006079648628.99466145
GATCCGG1606.836055E-527.1824916
TACGCTT1100.00967207226.3631174
Produced by FastQC (version 0.11.5)