FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1789_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1789_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233811
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC17670.143214803563917No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT13810.1119296229325237No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC701.6985156E-551.773872145
GCACCGT7000.036.2417146
TATACCG1051.8542499E-434.522915
ATTAACG850.002738224634.1278273
GCGAAAG3650.031.808362
AGCGAAA3750.030.9953481
AGCACCG8100.030.4313095
ACCGTGT8650.030.1665128
CGAAAGC3900.029.7524643
CACCGTG9450.029.1467747
GTATAGG3550.028.648871
CCGTGTT9650.028.5426969
GTGATCG2054.0485247E-728.2862139
CTGCCGC2954.838512E-1027.02772145
ATACCGA1400.001000926725.8869366
CTTAGAC4650.024.9536783
CCGACTA2101.5552761E-524.1670174
ATAGCAC10950.023.8427283
ACATAGC12250.023.1279071
CTAGACC3203.3867764E-822.6565784
Produced by FastQC (version 0.11.5)