FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_1789_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_1789_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2643749
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC2600.047.394634145
CTGCCGC10550.032.2923145
GCGAAAG11500.031.5585862
AGCGAAA11950.030.3983971
CGAAAGC12650.028.667913
TATAGAC13850.024.107572
TATACTG18600.021.826195
TGCGTAG2053.639808E-421.2443162
ATACCGC3259.855248E-720.0741626
GTATAGA29950.019.6488341
ATACCGT3001.1079639E-519.3306736
GTATAAG15450.018.8096241
ACCGTAC1950.00685114218.586138
GCGTAGT2359.180144E-418.5182483
GTATTAG11350.017.9230021
GTATAAT17550.017.8008171
GTATATA23350.017.735221
CCGTCCG3002.422352E-416.913389
GTATTAT28950.016.8141461
GTATAGG7601.8189894E-1216.2511441
Produced by FastQC (version 0.11.5)